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Second Complete Map of a Male Fruit Fly Brain Completed by Researchers

🔄 Updated 12d ago — new reporting from Tom's Hardware
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Key points

  • Connectome of a male fruit fly brain completed.
  • Collaboration between Janelia Research Campus and Google.
  • Provides a tool for neurobiology research.
  • Refines methods for mapping complex nervous systems.
  • Connectome includes the central nervous system.
  • Connectome contains over 166,000 neurons.
  • AI reconstructed 3D neural shapes from 2D images.
  • Software engineers trained the model to play Doom and Super Mario 64.
  • The connectome is named MaleCNS v1.0.
  • MaleCNS v1.0 released earlier this month.
  • Female fruit fly connectome released in 2024.
  • Connectome includes optic lobes and ventral nerve cord.
  • Reddit user trained the connectome to play Balatro.
  • Connectome achieved a 20% win rate in Balatro on lowest difficulty.
  • FutureBit released HashFly, a web-based proof-of-concept.
  • HashFly simulates Bitcoin mining using the fruit fly connectome.
  • FutureBit claims organic neurons could have 10x the efficiency of 3nm ASICs.
  • HashFly shows 2,914 neuron traces firing.
  • HashFly focuses on simulated photoreceptors and PPL101 cells.

Male Fruit Fly Brain Mapped

Researchers announced the completion of a connectome for a male fruit fly brain. This map details every neuron within the brain, offering a comprehensive view of its neural structure. This follows the earlier completion of a female Drosophila connectome this year.

Collaboration and Methodology

The project was a joint effort between biologists at the Howard Hughes Medical Institute’s Janelia Research Campus and computer scientists at Google. The collaboration was essential, as preparing the brain for high-resolution imaging and interpreting the resulting images required diverse expertise. Mapping hundreds of millions of synapses in a fruit fly brain is a task that cannot be completed by humans in a short timeframe.

Implications for Neurobiology

The completed connectome serves as a new tool for neurobiology research, potentially accelerating understanding of brain function. It also allows for comparative studies between male and female fruit fly brains. The techniques developed during this project are expected to be applied to mapping more complex nervous systems, including those of vertebrates.

Understanding Brain Function

A connectome helps neurobiologists understand how the brain processes information. Brain activity involves neurons communicating to transform sensory inputs into interpretable signals, route information to processing centers, and produce outputs such as memory formation or muscle movement.

Updates

🕒 2026-09-20 · new reporting from Tom's Hardware
  • FutureBit released HashFly, a web-based proof-of-concept.
  • HashFly simulates Bitcoin mining using the fruit fly connectome.
  • FutureBit claims organic neurons could have 10x the efficiency of 3nm ASICs.
  • HashFly shows 2,914 neuron traces firing.
  • HashFly focuses on simulated photoreceptors and PPL101 cells.
🕒 2026-09-18 · new reporting from 404 Media, Tom's Hardware
  • MaleCNS v1.0 released earlier this month.
  • Female fruit fly connectome released in 2024.
  • Connectome includes optic lobes and ventral nerve cord.
  • Reddit user trained the connectome to play Balatro.
  • Connectome achieved a 20% win rate in Balatro on lowest difficulty.
🕒 2026-09-08 · new reporting from Tom's Hardware
  • Connectome includes the central nervous system.
  • Connectome contains over 166,000 neurons.
  • AI reconstructed 3D neural shapes from 2D images.
  • Software engineers trained the model to play Doom and Super Mario 64.
  • The connectome is named MaleCNS v1.0.

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How outlets covered it

FutureBit released HashFly, a web-based proof-of-concept that simulates Bitcoin mining using a fruit fly connectome. This project explores the potential efficiency of organic computing for cryptographic hashing, with FutureBit claiming real organic neurons could offer significantly higher efficiency than current ASICs.

A Reddit user claims to have trained Google's recently released fruit fly brain simulation to play the game Balatro using a reinforcement learning algorithm, achieving a 20% win rate on the lowest difficulty. This development demonstrates an application of the fruit fly connectome in game AI, though details on the training methodology are limited.

HHMI Janelia Research Campus and Google Research released MaleCNS v1.0, a complete digital connectome of an adult male fruit fly brain, which has been used by online modders for various virtual tasks. This release follows the female fruit fly connectome from 2024 and enables detailed cross-sex comparisons of complex nervous systems. The connectome's viral spread highlights its potential as a research tool in neuroscience and AI, while also demonstrating public interest in simulated biological systems.

Google Research, in collaboration with HHMI Janelia Research Campus, has completed the first full connectome map of an adult male fruit fly's brain and central nervous system, comprising over 166,000 neurons. This achievement, aided by AI to reconstruct 3D neural shapes from 2D images, is considered a significant milestone in neuroscience. Following the announcement, software engineers have already begun training this fly-brain model to play video games like Doom and Super Mario 64.

Researchers from Howard Hughes Medical Institute’s Janelia Research Campus and Google have completed the connectome, a map of every neuron, in a male fruit fly brain. This achievement provides a new tool for neurobiology research and refines methods for mapping more complex nervous systems, building on a previous map of a female fruit fly brain.