A college microbiology lab class experienced an incident where 33 individuals, including students, professors, and lab workers, believed they had been exposed to a deadly germ. This led to 32 people beginning an antibiotic regimen as a precautionary measure, and two individuals underwent lumbar punctures after developing mild symptoms.
The bacteria in question was intended to be Plesiomonas shigelloides, a common and non-dangerous bacterium known to cause diarrhea in humans but also found widely in the environment and healthy individuals. However, every person in the class identified the mystery microbe as Neisseria meningitidis, a dangerous pathogen capable of causing meningitis and bloodstream infections.
Instructors found no fault with the students' conclusions, as their tests consistently identified the bacteria as a gram-negative, oxidase- and catalase-positive diplococcus with carbohydrate fermentation patterns matching N. meningitidis. This discrepancy highlights a significant issue in the identification process within the lab setting.
The incident, published in Morbidity and Mortality Weekly Report, serves as a cautionary tale regarding laboratory protocols and identification methods. N. meningitidis requires specialized handling, including respiratory protection and biosafety cabinets, which were not used by the students who only had gloves and lab coats.
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A college microbiology class, including students and staff, believed they were exposed to a deadly pathogen, leading 32 individuals to take antibiotics and two to undergo spinal taps. The incident, detailed in Morbidity and Mortality Weekly Report, revealed that the bacteria was actually a harmless strain, despite all lab tests indicating the dangerous pathogen.